MFE
primer
3.1
About 3.0
(current)
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Specificity
* Primer sequences (<= 50)
(example)
Number of primer sequences should <= 50, degenerate primers should <= 2.
* Background Database
C_elegans_GCF_000002985.6_WBcel235_genomic.fna
C_elegans_GCF_000002985.6_WBcel235_rna.fna
C_elegans_mRNA_and_gDNA.fna
Drosophila_melanogaster_GCF_000001215.4_Release_6_plus_ISO1_MT_genomic.fna
Drosophila_melanogaster_GCF_000001215.4_Release_6_plus_ISO1_MT_rna.fna
E_coli_OP50_genomic.fna
chrM.fa
hg38.fa
human_GCF_000001405.39_GRCh38.p13_genomic.fna
human_GCF_000001405.39_GRCh38.p13_rna.fna
human_rat_mouse_genomic.fna
human_rat_mouse_rna.fna
mouse_GCF_000001635.27_GRCm39_genomic.fna
mouse_GCF_000001635.27_GRCm39_rna.fna
rat_GCF_015227675.2_mRatBN7.2_genomic.fna
rat_GCF_015227675.2_mRatBN7.2_rna.fna
refseq_mito.fna
Allow mismatch at the 3' end
No
Yes
Accurate but slow, new in version 3.0.
T
m
Min
°C
Minimum T
m
value required for the binding stability between primer and its binding sites.
Size (bp)
From
To
Product size: 0 (min) - 100000 (max).
Concentration of monovalent cations (usually KCl)
mM
Concentration of divalent cations (usually MgCl
2
)
mM
Concentration of dNTPs
mM
Annealing oligo concentration
nM
MFEprimer-3.1
Featured
Specificity analysis
Dimer analysis
Hairpin analysis
Special Thanks / 特别感谢
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