MFE
primer
3.1
About 3.0
(current)
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Hairpin
* Primer sequences
(example)
Min loop size
Min
Max loop size
Max
Min double helix size
Min
Allowed max gaps for a hairpin
Max
Score
Min
Score cutoff value to filter hairpins
T
m
Min
°C
T
m
cutoff value to filter hairpins
ΔG
Max
kcal/mol
ΔG cutoff value to filter hairpins
Concentration of monovalent cations (usually KCl)
mM
Concentration of divalent cations (usually MgCl
2
)
mM
Concentration of dNTPs
mM
Annealing oligo concentration
nM
MFEprimer-3.1
Featured
Specificity analysis
Dimer analysis
Hairpin analysis
Special Thanks / 特别感谢
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