MFEprimer-3.0: PCR Primer Quality Control - Specificity, Hairpins & Dimers<

* Primer sequences (example)

Allowed max mismatches for a dimer

Max

Only output dimers with 3'end binded

Score

Min

Score cutoff value to filter dimers

T_m

Min

°C

T_m cutoff value to filter dimers

ΔG

Max

kcal/mol

ΔG cutoff value to filter dimers

Concentration of monovalent cations (usually KCl)

mM

Concentration of divalent cations (usually MgCl₂)

mM

Concentration of dNTPs

mM

Annealing oligo concentration

nM