MFE
primer
3.1
About 3.0
(current)
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Dimer
* Primer sequences
(example)
Allowed max mismatches for a dimer
Max
Only output dimers with 3'end binded
No
Yes
Score
Min
Score cutoff value to filter dimers
T
m
Min
°C
T
m
cutoff value to filter dimers
ΔG
Max
kcal/mol
ΔG cutoff value to filter dimers
Concentration of monovalent cations (usually KCl)
mM
Concentration of divalent cations (usually MgCl
2
)
mM
Concentration of dNTPs
mM
Annealing oligo concentration
nM
MFEprimer-3.1
Featured
Specificity analysis
Dimer analysis
Hairpin analysis
Special Thanks / 特别感谢
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